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Cloning Kits and Ligation


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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Supplier:  New England Biolabs (NEB)
Description:   SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA olig...
Small Business Enterprise
Supplier:  New England Biolabs (NEB)
Description:   The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-ta...
Small Business Enterprise
Supplier:  Promega Corporation
Description:   T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.

Supplier:  Promega Corporation
Description:   HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone a...
Supplier:  New England Biolabs (NEB)
Description:   Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum
Small Business Enterprise
Supplier:  New England Biolabs (NEB)
Description:   Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
MSDS SDS
Small Business Enterprise
Supplier:  New England Biolabs (NEB)
Description:   T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.
Small Business Enterprise
Supplier:  New England Biolabs (NEB)
Description:   T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.
Small Business Enterprise
Supplier:  New England Biolabs (NEB)
Description:   T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.
Small Business Enterprise
Catalog Number: (PAP2301)

Supplier:  Promega Corporation
Description:   NM522 contains an F' episome, which is required for production of ssDNA and for blue/white color selection.
Supplier:  New England Biolabs (NEB)
Description:   NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contai...
Supplier:  New England Biolabs (NEB)
Description:   T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.
Small Business Enterprise
Supplier:  New England Biolabs (NEB)
Description:   EnGen® Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from streptococcus pyogenes wit...
New Product
Supplier:  New England Biolabs (NEB)
Description:   T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity
Small Business Enterprise

Supplier:  Promega Corporation
Description:   The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.
Supplier:  Promega Corporation
Description:   For ligation of DNA ends, mutagenesis and cDNA cloning.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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